Molecular Cloning A Laboratory Manual


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Molecular Cloning: A Laboratory Manual
20 August 2011

Molecular Cloning: A Laboratory Manual
Molecular Cloning: A Laboratory Manual
English | PDF | 2344 pages | 653.43 MB

The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using todays biomolecular techniques. The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.
Molecular Cloning: A Laboratory Manual
27 August 2011

Molecular Cloning: A Laboratory Manual

Molecular Cloning: A Laboratory Manual
English | PDF | 2344 pages | 653.43 MB
Laboratory Exercises in Organismal and Molecular Microbiology
3 February 2012

Laboratory Exercises in Organismal and Molecular Microbiology

Laboratory Exercises in Organismal and Molecular Microbiology
English | 2003-03-27 | ISBN: 0072487445 | 384 pages | PDF | 60.1 mb

A modern general microbiology laboratory manual that combines the procedural details of a laboratory manual with the photographic support of a laboratory atlas. The 46 class-tested laboratory experiments are divided into 9 specialty areas, and the extensive four-color illustration program includes 220 photos and micrographs plus 150 line drawings.
US Coast Guard - Boat Crew Seamanship Manual
1 January 2011

US Coast Guard - Boat Crew Seamanship Manual

US Coast Guard - Boat Crew Seamanship Manual
English | Publisher: Progressive Management (July 16, 2010) | ISBN-10: 1422051927 | 256 pages | PDF | 22.58 MB


The bonus Coast Guard manuals collection includes: 25 Foot Transportable Port Security Boat Operator's Handbook, 47 Ft Motor Lifeboat Operator's Handbook, Auxiliary Aviation Program, Auxiliary Boat Crew Training Manual, Auxiliary Manual, Auxiliary Operations Policy Manual, Aviation Life Support Systems Manual, Boat Crew Seamanship Manual, Boat Force Operations Personnel Qualifications Standard, Boat Management Manual, Bridge Administration Manual, Civil Engineering Manual, Coast Guard Air Operations Manual, Coast Guard Aviation Medicine Manual, Coast Guard Diving Policies And Procedures Manual, Volume I, Coast Guard Helicopter Rescue Swimmer Manual, Coast Guard Medical Manual , Cutterboat Operator's Handbook, Defender Class Operator's Handbook, Electronics Manual , Emergency Medical Services Manual , Joint Staff Officers Guide, Armed Forces Staff College (Afsc),
Gene Cloning and DNA Analysis: An Introduction (Brown, Gene Cloning and DNA Analysis)
24 June 2010

Gene Cloning and DNA Analysis: An Introduction (Brown, Gene Cloning and DNA Analysis)


Gene Cloning and DNA Analysis: An Introduction (Brown, Gene Cloning and DNA Analysis)


Wiley-Blackwell | 2010-04-26 | ISBN: 1405181737 | 336 pages | PDF | 16 MB



Known world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations.
Molecular Profiling: Methods and Protocols
29 February 2012

Molecular Profiling: Methods and Protocols

Molecular Profiling: Methods and Protocols

2012 | 460 | ISBN: 1603272151 | PDF | 6 Mb

The next revolution in molecular medicine is the application of molecular profiling to individualized patient therapy. Molecular profiling technology has advanced dramatically, particularly in the field of cancer tissue biomarkers. It is now possible to gather complex genomic and proteomic information from a routine clinical needle biopsy or surgical specimen. In Molecular Profiling : Methods and Protocols, expert researchers in the field focus on the entire process from discovery to commercialization, with practical guides that are not limited to experimental methods. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as essays and guidelines for grants, patents, and commercialization of products related to molecular profiling. Authoritative and practical, Molecular Profiling: Methods and Protocols seeks to aid scientists in understanding the latest advancements in genomics, proteomics, imagining, and bioinformatics....
Current Advances in Molecular Mycology
28 March 2010

Current Advances in Molecular Mycology
Current Advances in Molecular Mycology By Youssuf Gherbawy
Nova Science Publishers 2009 | 395 Pages | ISBN: 1604569093 | PDF | 21 MB

Molecular mycology has been playing a pivotal role in 21st century. It is emerging with full impact. It is multi-disciplinary and includes molecular markers, recombinant DNA techniques, cloning, phylogeny and bioinformatics. Varying in application of concepts, practice, scale, style and substance, molecular mycology is amongst the latest globalising frontiers of the corporate world. This branch is being regarded as a core subject in many colleges and universities. In the book, various topics on molecular mycology are uniquely combined to provide a complete overview of the subject. The book addresses the role of molecular and bioinformatics tools in solving the problems of identification of fungi and discusses current trends in Molecular Mycology.
Molecular Pathology Protocols (Methods in Molecular Medicine) by Anthony A. Killeen
6 September 2012

Molecular Pathology Protocols (Methods in Molecular Medicine) by Anthony A. Killeen

Anthony A. Killeen, "Molecular Pathology Protocols (Methods in Molecular Medicine)"
ISBN: 1617371319 | edition 2010 | PDF | 456 pages | 3 mb


Anthony Killeen has assembled a collection of readily reproducible molecular pathology techniques that are either frequently performed or recognized for their significant diagnostic utility. Each method is described in step-by-step detail by a leading molecular pathologist or laboratory scientist who has developed it or used it extensively.
Laboratory Manual for Principles of General Chemistry, 9 edition
26 August 2011

Laboratory Manual for Principles of General Chemistry, 9 edition


Laboratory Manual for Principles of General Chemistry, 9 edition by Jo Allan Beran


W..ey | 2010 | ISBN: 0470647892 | 464 pages | PDF | 83 MB



This new edition of the Beran lab manual emphasises chemical principles as well as techniques. The manual helps students understand the timing and situations for the various techniques. The Beran lab manual has long been a market leading lab manual for general chemistry. Each experiment is presented with concise objectives, a comprehensive list of techniques, and detailed lab intros and step-by-step procedures.
Molecular Hematology by Drew Provan
28 July 2010

Molecular Hematology by Drew Provan

Molecular Hematology by Drew Provan
Publisher: Wiley-Blackwell; 2 edition (February 18, 2005) | ISBN: 1405112557 | Pages: 344 | PDF | 6.96 MB

The highly specialized functions of blood cells and their ready accessibility account in large part for the rapidly expanding knowledge of the molecular basis of hematologic diseases. In his foreword to this book, Max Perutz states that the day he spent trying to correlate amino acid substitutions in the globin molecule with the symptoms they may cause was "one of the most exciting days" in his scientific life. In Molecular Haematology, Provan and Gribben aim to show the ways in which molecular biology has profoundly influenced our understanding of blood diseases and the ways in which molecular techniques can be used in the diagnosis and treatment of these disorders. The contributions cover the main fields of hematology, from inherited diseases to neoplasia. Several chapters deal with diagnostic techniques (molecular cytogenetics and the detection of minimal residual disease) and treatment (hematopoietic growth factors and gene therapy). Readers without a strong background in molecular biology will find detailed explanations, numerous diagrams, and an overview of recent advances in cancer-cell biology, with particular emphasis on leukemia and lymphoma.